摘要
对福眼龙眼果皮的过氧化物酶(POD)进行分离纯化,得到了一组包含4个不同分子质量的POD.
Peroxidase (POD) was extracted from the peels of matural longan (Dimocarpus longan Lour.cv.Fuyan) fruits. The extraction was precipitated with ammonium sulfate between 30% and 80% saturation. And, the enzyme solution was desalted by Sephadex G-25 column chromatography, separated by DEAE-Cellulose column chromatography, hydroxyapatite (HA) column chromatography and Sephacryl S-200 HR column chromatography respectively. Four fractions of POD whose molecular mass are 290.1, 232.1, 188.5 and 170.7 ku were detected with the silver stain after 5%-20% semi-dry native linear gradient flatted polyacrylamide gel electrophoresis. POD and polyphenol oxidase (PPO) showed no difference in native polyacrylamide gel electrophoresis. Moreover, the purified POD showed the activities of PPO in our preliminary study.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2003年第4期543-544,共2页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家"948"计划项目(991029).
