摘要
以红豆杉带侧芽茎段为外植体进行离体繁殖.结果表明:在WPM、MS、B53种基本培养基中,WPM为红豆杉离体培养最适的基本培养基;初代培养的最适培养基为WPM+4.0mg·L-12-ip+30.0mg·L-1Ad;芽继代增殖的最适培养基为WPM+4.0mg·L-12-ip+1.0mg·L-1IAA;生根壮苗的最适培养基为1/2WPM+0.5mg·L-1IBA+0.1mg·L-1NAA.
Propagation for yew (Taxus chinensis var.mandiya) was studied using stem segments with lateral buds as explants. The results showed that the optimal media were WPM for basic in vitro culture, WPM+4.0 mg·L^(-1) 2-ip+30.0 mg·L^(-1) Ad for primary culture, WPM+4.0 mg·L^(-1)2-ip+1.0 mg·L^(-1) IAA for subculture, 1/2WPM+0.5 mg·L^(-1) IBA+0.1 mg·L^(-1) NAA for the growing of roots and seedlings.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2003年第4期482-485,共4页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
关键词
红豆杉
带侧芽茎段
离体培养
植株再生
yew (Taxus chinensis var.mandiya)
stem segment with lateral bud
in vitro culture
plant regeneration
