摘要
通过试验,筛选出对水貂肠道冠状病毒敏感的貂肺传代细胞系(ML),经胰酶处理后,从6份病貂粪样中分离获得2株冠状病毒.培养时所用的犊牛血清也作了一定处理.该病毒粒子经电镜观察,多呈不规整的圆形,直径80～160nm,有囊膜,外周有花瓣样突起,经鉴定为RNA病毒.对乙醚敏感.37℃12h可失去感染性.该病毒在pH3条件下室温3h仍保持一定活力.该病毒接种健貂可引起部分貂出现轻度腹泻.
Two strains of coronavirus were isolated from 6 fecal samples of infected minks with mink lung cell line (ML) cultures. Its morphological features visualized by electron microscopy: Most enveloped particles are irregular round with average diameter ranging from 80 to 160 nm. Petal-shaped surface projections protrude from envelope. It is ether-sensitive and does not tolerate to 37℃ for 12 hours, but remains a certain viability at room temperature for 3 hours in pH3. 0 medium. It was identified as mink enteric coronavirus. In inoculation test, some of minks experimentally infected with the virus were sick and manifested diarrhea.
关键词
肠道
水貂
冠状病毒
分离
鉴定
mink
coronavirus
isolation and idenfication
