摘要
应用光镜和免疫组化的方法,研究27例小细胞肺癌(SCLC)的光镜分型和3类共11种蛋白性标记物的表达。发现SCLC的NSE、Chromogranin、Synaptophysin、S-100的表达率分别为81.48％、11.11％、44.44％和18.52％;Cytokeratin、Vimentin、NF的表达率为92.59％、14.81％和22.22％;Desmin、GFAP未见表达。说明SCLC具有神经-上皮性分化。SCLC的Serotonin和Somatostatin的表达率14.81％和44.44％,表明其具有激素分泌的特性。综合应用上述抗体组合,SCLC的准确诊断率可达96％,具较高的准确性和应用价值。尤可用于治疗与复发的监视和转移灶的辨认。
Twenty seven cases of small cell lung cancer (SCLC)were studied microscopically and immunohistochemically for their histological typing and expression of 11 antigens in 3 groups. We found that the expression percentage of NSE, Chromogranin, Synaptophysin, S- 100, Cytokeratin, Vimentin and NF were 81.48%, 11. 11%, 44.44%, 18. 52%, 92. 5%, 14. 81% and 22.22% respectively. No SCLC was found to express Desmin and GFAP. All these foundings indicate that SCLC has the feature of neuronepithelial eifferentiation. The expression ratio of serotonin and somatostatin were 14.8% and 44.44%, respectively sho- wing that SCLC has the function of hormone secretion. Combining application of above anti- bodies, an accurate diagnosis of about 96% SCLC cases could be reached.
关键词
免疫组织化学
神经内分泌
肺肿瘤
small cell lung cancer
immunohistochemistry
intermediate filment
neuroendocrine
polypeptide hormone
