摘要
从分离到的乳酸菌中筛选到1株共轭亚油酸高产菌株,乳杆菌L1(Lactobacillussp.),在MRS培养基上,乳杆菌L1产生共轭亚油酸的最适亚油酸的浓度是0.1%(v/v),最适培养时间是42h,参与共轭亚油酸形成的酶是胞内酶,并且可能是由多种酶共同作用的结果。建立了共轭亚油酸的紫外光谱扫描和高效液相色谱检测方法。
A high conjugated linoleic acid (CLA) producer, Lactobacillus L1 (Lactobacillus sp.) was screened from 36 strains of lactic acid bacteria. The optimum conditions for CLA production in MRS medium were that concentration of linoleic acid was 0.1%(v/v), incubation time was 42hs. The enzyme that formed CLA was intracellular. It could be a multienzyme reaction for the formation of CLA from linoleic acid. We also set up a UV-scan and high performance liquid chromatography methods to determine CLA in this research.
基金
江西省教育厅科研基金(NO:2001387)"酶法生产共轭亚油酸的研究"的部分内容
关键词
共轭亚油酸
乳酸菌
筛选
发酵条件
高产菌株
Conjugated linoleic acid (CLA)
lactic acid bacteria
process conditions
