摘要
目的 观察低剂量顺铂对肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导细胞凋亡的增敏作用。方法 人前列腺癌细胞株LNCaP经过TRAIL(100ng/ml)、低剂量顺铂(0.2～1.0mg/ml)以及联合用药处理24h后,观察其生存率以及相关蛋白的动态变化。结果 LNCaP细胞株单独经过TRAIL或低剂量顺铂处理后并不能降低其存活率,但联合用药后细胞生存率大大降低。经1.0mg/ml顺铂处理后的LNCaP细胞株,其凋亡抑制蛋白BCL-2的表达随时间延长而明显下降。结论 低剂量顺铂可以增强TRAIL对前列腺癌细胞的杀伤效应,效果优于两种药物的单独使用;凋亡抑制蛋白BCL-2可能是肿瘤细胞抵抗TRAIL诱导凋亡机制中的一个重要因素。
Objective To determine the synergistic effect of low dose cis - diaminedichloroplatin ( C - DDP) on augmenting tumor necrosis factor related apoptosis - inducing ligand ( TRAIL) - induced apoptosis in human prostate cancer cell line LNCaP. Methods LNCaP cells were treated with TRAIL a-lone(100ng/ml) , C -DDP alone (0.2-1. 0mg/ml) or in combination for 24h. Cell viabilities and apop-totic inhibitory proteins were determined by flowcytometry and immunoblot respectively. Results Apoptosis in LNCaP cells was dramatically enhanced by the treatment of TRAIL in combination with C - DDP, but not by either of them alone. BCL - 2 , one of apoptotic inhibitory proteins, was down regulated under C - DDP treatment. Conclusion Combination treatment of LNCaP cells with TRAIL and low dose C -DDP could enhance the cell sensitivity to TRAIL. Moreover, anti - apoptotic protein BCL -2 is proposed to be an important agent in TRAIL - induced apoptosis in LNCaP cells.
出处
《上海第二医科大学学报》
CSCD
2003年第B10期25-27,37,共4页
Acta Universitatis Medicinalis Secondae Shanghai
