摘要
目的 观察 2 0 1A中药合剂防治大鼠抗肾小球基底膜 (GBM)肾炎的疗效。方法 建立大鼠抗GBM肾炎模型。实验分 3组 :2 0 1A处理组、肾炎对照组及正常对照组。 2 0 1A处理组大鼠一次性尾静脉注射抗GBM抗血清后即刻给予 2 0 1A合剂 (0 .42g/kg ,灌胃 ) ,至第 2 1天。对照组则给予等量的生理盐水。定期于第 4天、第 14天和第 2 1天 ,检测大鼠尿蛋白 ,观察肾组织病理学改变。结果 肾炎对照组大鼠注射抗血清后于第 4天即出现异常蛋白尿 ,肾小球内可见细胞数增加和新月体形成 ,肾小管内大量蛋白管型 ,GBM呈不规则增厚。而 2 0 1A处理组鼠上述病变均明显好转。结论 2 0
Objective To inves tigate t he preventive effect of 201 A on the development of anti-GBM nephritis in rats. ?Methods The anti-GBM nephritis model was established by injecting rabbit anti-rat GBM antibody in SD rats. The model rats were divided into 3 groups: treatment group (with 201A 0.42 g·kg -1 ·d -1 ), untrea ted nephritis control group and normal control group. Urinary protein was measur ed on 4d, 14d and 21 d. Kidney specimens were collected at intervals for patholo gical study.?Results Heavy proteinuria developed 4 d af ter the injection of anti-GBM antibody. Glomerular hypercellularity, crescents a nd protein casts were found in the nephritic rats. Electron microscopy showed th ickening of GBM and loss of foot processes. All of the biochemical and pathologi cal changes were evidently less serious in the 201 A treated group than in the u ntreated nephritis control group.?Conclusion 201 A can inhibit the development of anti-GBM nephritis in rats.
出处
《徐州医学院学报》
CAS
2003年第6期488-491,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省科委及卫生厅自然科学基金赞助(BK97154
H972 3 )
