摘要
利用适用于禾谷类作物的表达载体pGU4ABBar ,采用花粉管通道法 ,将人工合成的苏云金芽孢杆菌杀虫蛋白基因cryIa导入了优良冬小麦品系西农 2 2 0 8和西农 132 ,经PCR和Southernblot鉴定 ,证明获得了 2 7株导入了cryIa基因的转基因植株 ,转化率约为 1.13%～ 1.2 1% ,并通过Westernblot鉴定检测到了目的蛋白的表达。
The cryIa gene, a synthetical insecticidal crystal protein gene of Bacillus thuringiensis, was transferred into the wheat varieties Xinong 2208 and Xinong 132 using the gramineous expression vector pGU4ABBar by pollen-tube pathway. By PCR and Southern blotting analysis 27 transgenic plants with cryIa gene were obtained. Western blotting analysis showed that the protein was expressed in the transgenic plants. The transformation frequency was about 1.13%-1.21%.
出处
《作物学报》
CAS
CSCD
北大核心
2003年第6期806-809,共4页
Acta Agronomica Sinica
基金
"九五"国家重点科技攻关资助项目 (专题 ) (96 0 0 9 0 1 14 )
杨凌农业生物技术育种中心开放课题资助项目 (1999 19)
