摘要
目的:研究端粒酶在结核性腹膜炎腹水与恶性腹水的活性水平,探讨腹水端粒酶检测鉴别结核性腹水与恶性腹水的诊断价值。方法:应用TRAP-PCR-ELISA方法和TRAP-PCR-银染法分别对13例结核性腹膜炎和18例恶性腹水标本(肝癌7例,胃癌6例,结肠癌2例,胰腺癌1例,卵巢癌2例)进行定性、定量端粒酶活性检测。对比腹水细胞学检查,分析腹水端粒酶活性鉴别结核性腹膜炎与恶性腹水的诊断价值。结果:恶性腹水组端粒酶活性(0.387±0.023)高于结核性腹膜炎腹水组(0.223±0.004),统计差异显著(P<0.01);恶性腹水端粒酶阳性率88.9％(16/18)明显高于结核性腹膜炎腹水组7.7％(1/13),P<0.01。仅1例结核性腹膜炎腹水端粒酶阳性。结论:恶性腹水端粒酶阳性,结核性腹水端粒酶阴性。腹水端粒酶活性检测可能是鉴别结核性与恶性腹水的重要依据。
AIM: To determine telomerase activity of exfoliated cells in tuberculous peritonitis and malignant ascites, and study the diagnostic value of telomerase activity in differentiating tuberculous peritonitis from malignant ascites. METHODS: TRAP-PCR-ELISA and TRAP-PCR-silver stdining were employed to determine telomerase activity in 18 specimens of malignant ascites and 13 specimens of tu- berculous peritonitis. Telomerase activities in tuberculous peritonitis and malignant ascites were analysed. RESULTS: Telomerase activity in malignant ascites (0.387±0.023) was significantly higher than that in tuberculous peritonitis (0.023±0.004, P<0.01). The positive rate of telomerase activity in malignant sscites was significantly higher than that in tuberculous peritonitis, 88.9% (16/18) vs 7.7% (1/13), P<0.01. The sensitivity, specificity and accuracy of determination of telomerase activity in diagnosis of ma- lignant ascites were 88.9%, 92.3%, and 90.3%, respectively. CONCLUSION: Telomerase activity is positive in malignant ascites and may serve as a useful indicator for differentiating between tuberculous peritonitis and malignant ascites.
出处
《世界华人消化杂志》
CAS
2003年第10期1563-1565,共3页
World Chinese Journal of Digestology
基金
辽宁省科学基金
No.2001225002-2
