摘要
对猪瘟RT PCR诊断方法与兔体交叉试验诊断方法的相关性进行了研究。通过计算机软件设计了猪瘟病毒E2基因保守区的 1对引物 ,建立了猪瘟病毒RT PCR检测方法 ,同时应用兔体交叉试验进行对比 ,对 7份临床送检病例进行了检测。结果表明 ,二者的符合率为 10 0 % ,但RT PCR可大大降低检测所用的时间 ,更便于猪瘟的快速诊断。
Relationship between RT-PCR and rabbit-cross test for detection of classical swine fever virus (CSFV) was studied in this experiment. One set of specific primers were designed according to the conserved sequence of E2 gene of CSFV in GenBank and a standard RT-PCR was founded to detect the infection of CSFV. Compared with rabbit-cross test, 7 samples from pigs suspected infection with CSFV were detected with RT-PCR. It was indicated that the results from the detection of CSFV by RT-PCR coinside completely with that from the detection with rabbit-cross test, and that RT-PCR detection can save plenty of time.
出处
《中国兽医科技》
CSCD
北大核心
2003年第11期16-21,共6页
Chinese Journal of Veterinary Science and Technology
基金
黑龙江省科委基金资助项目 (GB0 1B10 5 6 0 2 )
