摘要
本研究采用细胞化学和MTT定量的方法,观察鸡胚前脑神经细胞及其上清液(S_1)对小鼠腹腔巨噬细胞的作用。发现神经细胞及S_1可使培养的巨噬细胞酸性磷酸酶(AcP_(ase))和三磷酸腺苷酶(ATP_(ase))含量增加。MTT定量的结果表明在神经细胞及S_1作用下,巨噬细胞OD值比对照组明显高。S_1经各种处理后,进一步观察对巨噬细胞的影响,发现影响巨噬细胞活性的物质,很可能是一种膜糖蛋白。
Adult mouse peritoneal macropbagcs were allowed to co-culture with neuronal cells dissociated from the forebrain of E8 chick embryo or with their supernatant. It was observed that these activated macrophages are capable of phagocytosing some of the neuronal cells. Results of the cytochemical stainings for activities of acid phosphatase and adenosine triphosphatase showed that both enzymatic activities are significantly higher in the co-cultures or/and in macrophage cultures incubated with the neuronal cell supernatant, than in the control cultures of macrophages or neuronal cells. A MTT automated colorimetric microassay, measuring mitochondrial dehydrogcnase of viable cells was used to quantify the activation of macrophages by an Elisa reader. The results revealed that low density cultures (<2x10^4 per well) arid high density cultures (<2x10^4 per well) of macrophages were activated 3 to 6 folds and 2 to 2.5 folds, respectively, by both neuronal cells or their supernatant. Further studies of the supernatant revealed that a macrophage activating factor which is alkaline and temperature (60℃, 1 hr) insensitive but acid, trypsin and neurao minidasc sensitive, exists in the neuronal cultures and in their supernatant.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1989年第4期245-249,327,共5页
Chinese Journal of Anatomy
基金
香港大学
陈蕉琴和医学院研究基金
关键词
神经细胞
巨噬细胞
上清液
鼠
macrophage, neuronal cell, AcP_(ase), ATP_(ase).
