摘要
本实验用刀豆球蛋白 A-辣根过氧化物酶胶体金(Con A-HRP-G)法定量分析了戊二醛固定后的人外周血白细胞表面受体的分布和密度,可见所有白细胞以及血小板均显示具有 Con A受体。细胞表面平均每μm 周长金标 Con A 复合物的定量分析表明,细胞表面 Con A 受体标记密度因细胞类型而不同。各类血细胞 Con A 受体的标记密度依次为:分叶核中性粒细胞<单核细胞>NK 细胞>杆状核中性粒细胞>嗜碱粒细胞>嗜酸粒细胞>淋巴细胞。小淋巴细胞的密度明显多于大淋巴细胞。NK 细胞表面的受体有极性分布。以上结果说明:1.Con A 受体与细胞功能活性有关,细胞越活跃,则其表面 Con A 受体越多;2.Con A 受体可能与细胞的识别功能有关,如NK 细胞 Con A 受体呈极性分布的现象;3.Con A 受体数目与细胞的成熟程度有关,细胞越成熟,其表面 Con A 受体越多。
Con A-colloidal-gold-labelled horseradish peroxidase was used to analyse the
density and distribution of concanavalin-A (Con A) receptor sites on the surface of
isolated human peripheral blood leucocytes.The quantitative analysis of the number
of gold Con A complex per micron of leucocyte surface indicated that the extent
of surface labeling was dependent on cell types.The extent of surface labeling from
more to less was:segmented neutrophil>monocyte>NK cell>band neutrophil>
basophil>eosinophil>lymphocyte,and especially the small lymphocytes were more
heavily labeled than the larger ones,and the Con A receptor of NK cell exhibited
polarization along the cell surface.These results suggest that:1.the extent of Con
A receptors was related to the functional activities of the ceils,the more active the
cell is,the more the Con A receptors will be;2.the receptors of Con A were
probably related to the cell recognition,for instance,on the NK cell Con A rece-
ptors showed polar distribution;3.the number of receptors of Con A was dependent
on the cell differentiation,the more mature the cell is,the more the Con A
receptors are.
出处
《解剖学报》
CAS
CSCD
北大核心
1989年第4期405-409,共5页
Acta Anatomica Sinica
关键词
胶体金
外周血细胞
刀豆素A
定量分析
定位分析
Receptor
Leucocyte
Concanavalin A
Colloidal gold
Electron microscopy
NK cell
