摘要
应用 Con A-HRP-G 二步标记法,对健康人新鲜精子及经棉酚处理后的精子作了标记,并在光镜、透射电镜及扫描电镜下作了对比观察。结果表明,在人的精子的质膜表面,从头至尾均有金粒标记。以头部及尾部中段较为致密。在被棉酚破坏了质膜的精子内部,在顶体膜及残破的线粒体内也出现金标记。说明不仅在精子的质膜表面,而且在顶体膜及线粒体等内膜系统,都有Con A 受体。本文还讨论了该受体在人精子存在的意义,以及 Con A-HRP-G 二步标记法和光镜、透射电镜、扫描电镜对比观察法的优点。
Intact and gossypol-treated spermatozoa from healthy man were labeled with
Con A-HRP-G two-step method.Comparative observations of these sperms were done
under LM,TEM and SEM.Results show that there are gold particles on sperm
surface membrane.Their distribution is more dens on plasma membrane of sperm
head and middle piece.On the intracellular membrane structures of sperm which plasma
membrane was damaged by gossypol the gold particles also can be seen,especially on
the membranes of acrosome and mitochondria.It was proved that Con A receptors
are present not only on plasma membrane of sperm,but also on the membrane of
acrosome and mitochondrion.The significance of Con A receptors on sperm struc-
tures,advantages of Con A-HRP-G two-step labeled method and comparative
observations under LM,TEM and SEM were discussed.
出处
《解剖学报》
CAS
CSCD
北大核心
1989年第1期79-82,共4页
Acta Anatomica Sinica
关键词
人
精子
Con受体
胶体金
Human sperm
Con A receptor
Colloidal gold
Gossypol
