摘要
目的：建立测定血清和尿液中司帕沙星的反相高效液相色谱法。方法：用ＨＰＬＣ法测定了４ 例患者单剂量口服司帕沙星后血清和尿中药物浓度。结果：司帕沙星色谱保留时间ＴＲ 为８．３３ｍｉｎ。在０ ．０５～２ｍｇ·Ｌ－ １ 和０．１～４ｍｇ·Ｌ－１ 范围内，血清和尿药物浓度与色谱峰高呈良好线性相关。结论：建立了测定体内司帕沙星药物浓度的方法。
AIM:To developed a reversed phase high performance liquid chromatography(RP-HPLC) method for the determination of sparfloxacin in serum and urine.METHODS The sparfloxacin's concentrations in four patients' serum and urine were determined and the pharmacokinetics parameters were computed after a single oral of 400 or 200mg sparfloxacin tablet. RESULTS The chromatography retention time of sparfloxacin was at 8.33min. The standard curve was linear in the concentration range of 0.05～2mg·L -1 in serum and 0.1～4mg·L -1 in urine. CONCLUSION The results showed this RP-HPLC method was good for determining sparfloxacin in body fluid.
出处
《解放军药学学报》
CAS
1999年第3期40-42,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
