摘要
目的 在SOF +PVA(合成输卵管液 +聚乙烯醇 )这一化学成分明确培养系统条件下 ,观察了葡萄糖、丙酮酸和乳酸三种碳水化合物对牛体外受精胚胎体外发育的影响 ,以便为今后进一步探讨影响牛早期胚胎体外发育的因素提供实验依据。方法 牛卵母细胞体外成熟和体外受精后 ,在化学成分明确培养系统内进行体外发育培养。结果 实验 1将牛体外受精卵培养于不含有葡萄糖的SOF +PVA培养系统中 ,培养 12 0h后分别移入含有 0、1 5 0、3 30、5 0 0mmol L的SOF +PVA培养系统中 ,对照组胚胎一直在含有 1 5 0mmol L葡萄糖的SOF +PVA中培养 ,结果囊胚的发育率分别为 9 2 % a、12 1%、19 2 % b、18 9%和 11 7% (a <b ,P <0 0 5 )。实验 2将牛体外受精卵分别培养于含有 0、0 10、0 2 4、0 33和 0 6 6mmol L丙酮酸钠的SOF +PVA培养系统中 ,结果囊胚的发育率分别为 9 8%、15 7%、11 3 %、12 9%和 11 9%。实验 3将牛体外受精卵分别培养于含有 0、1 98、3 30、4 79和 5 87mmol L乳酸钠的SOF +PVA培养系统中 ,结果囊胚的发育率分别为 2 0 6 %、2 3 2 %、18 4%、2 1 6 %和 19 3%。结论 在该培养系统条件下 ,培养 12 0h后再加入葡萄糖与培养系统内一直添加有葡萄糖相比更有利于胚胎的发育 ;培养系统中单独缺乏丙酮酸或?
Objective The effects of glucose, pyruvate and lactate on in vitro development of bovine embryos produced in vitro in chemical defined culture system (SOF+PVA) were observed in this research. Method Bovine oocytes were matured and fertilized in vitro, then the fertilized ova were cultured in vitro in chemical defined culture medium. Results Experiment 1: Bovine IVF embryos were cultured in SOF+PVA that was not supplemented with glucose, then were cultured in SOF+PVA supplemented with 0, 1.50, 3.30 and 5.00mmol/L glucose respectively after being cultured 120 h, the embryos in control group were cultured in SOF+PVA supplemented with 1.50 mmol/L glucose during all the culture time. The results showed that the development rates of blastocysts were 9.2% a, 12.1%, 19.2% b, 18.9% and 11.7% respectively (a<b, P<0.05). Experiment 2: Bovine IVF embryos were cultured in SOF+PVA supplemented with 0, 0.10, 0.24, 0.33 and 0.66mmol/L sodium pyruvate respectively, the development rates of blastocysts were 9.8%, 15.7%, 11.3%, 12.9% and 11.9% respectively. Experiment 3: Bovine IVF embryos were cultured in SOF+PVA supplemented with 0, 1.98, 3.30, 4.79 and 5.87mmol/L sodium lactate respectively, the rates of blastocysts were 20.6%, 23.2%, 18.4%, 21.6% and 19.3% respectively. Conclusion It is more beneficial for embryo development that glucose is supplemented in culture system after being cultured 120h than that supplemented in culture system during all the culture time under this condition. There is no effect on the in vitro developmental rates of embryos when pyruvate or lactate is absent in culture system.
出处
《中国实验动物学报》
CAS
CSCD
2003年第1期38-41,共4页
Acta Laboratorium Animalis Scientia Sinica
基金
内蒙古自然科学基金项目资助课题 ( 9910 4- 2 )
关键词
碳水化合物
受精
胚胎
牛
发育生物学
体外发育
Carbohydrates
Fertilization in vitro
Embryo, Cattle
Development Biology
