摘要
目的 探讨建立SD大鼠移植性肝癌模型的可行性。方法 用腹腔肝癌移植种鼠 (W2 56 2k)含癌细胞之腹腔液注入SD雄性幼鼠腹腔内行保种传代 ;同时行SD雄性幼鼠腋下及腹股沟皮下注射种植 ,7天后将皮下种植成功的大小约 1cm× 1cm× 1 .5cm实体瘤组织 ,切成 0 .2cm× 0 .2cm× 0 .3cm大的组织块 ,并植入SD成年雄性大鼠肝脏内制成移植性肝癌模型 ,于 7、1 0、1 3d分别处死大鼠 ,观察并记录模型鼠移植肝癌的大小、重量。结果 移植性肝癌模型成功率为 1 0 0 % (82 / 82 ) ,肿瘤自然消退率为零 (0 / 82 ) ,皮下接种成功率为 1 0 0 % (1 5/ 1 5) ,7天后 ,皮下接种的实体瘤平均重约 2 0 0mg ,而模型鼠的移植肝癌平均重约 39mg。结论 SD大鼠实验模型经济 ,其模型建立成功率高 ,肿瘤自然消退率低 。
Objective To study the feasibility of the establishment of transplantated hepatic cancer models in SD rats. Methods The male weaning SD rats were inoculated and transgenerated from the celiar transplantation tumor rats (W 256 2k ) by intraperitoneal injection of abdominal fluid of tumor cells, another weaning SD rats were prepared for the models of solid tumor by subcutaneous inoculation of the abdominal fluid of tumor cells. The solid tumors were cut to pieces of 0.2 cm×0.2 cm×0.3 cm,which were implanted into the liver of the adult male SD rats, and the transplantation hepative tumor models were established. After 7 days, the volume and weight of tumor mass was measured. Results The successful rate of model was 100% (82/82), the natural extinctive rate was 0 (0/82), the successful rate of inoculation was 100% (15/15). Conclusion SD rats are economical, the successful rate of model was high, the natural extinctive low, so the SD rats are ideal selection in establishing transplantation liver cancer model.
出处
《中国普外基础与临床杂志》
CAS
2003年第2期128-130,共3页
Chinese Journal of Bases and Clinics In General Surgery
