摘要
为了揭示鸡骨型白血病(下称OP)的X线阳性病变与其超微病理变化之间的关系,采用人工诱发与天然自发的病例作研究对象,经X线胫骨摄片确定OP诊断后,选择有代表性的30例,按诊断结果分成5组:OP轻度阳性(+)14例,中度阳性(++)4例,重度阳性(+++)6例,OP可疑(±)3例,阴性(-)对照3例。取胫骨中段骨组织作超薄切片行透射电镜检查对比,以观察两者的相关性。结果3例X线OP阴性鸡电镜所见为正常,骨细胞和哈佛氏管等超微结构无异常改变。X线OP可疑组和各阳性(+、++、+++)组,每组均检出病毒粒子,其大小形态与超微结构都与C型肿瘤病毒相似,为放射学诊断提供了病原证据。X线诊断为OP可疑和各组阳性的病例电镜观察全部有超微结构上的改变,包括骨细胞破坏、骨陷窝间隙增宽、骨质毛细血管病变等等数十种超微变化,表明两者具有一致相关性,为OP的放射诊断提供了超微病理学上的依据。
After being examined by radiology, 30 chickens with induced and natural osteopetrosis (OP) were selected for electron microscopy to make clear the relationship between radiological lesions and ultrastructural appearances. Fourteen cases were positive ( + ) , 4 cases were moderate ( + + ) , 6 cases were severe (+ + +), 3 cases were suspicious (±) , and 3 cases were negative ( - ) control. Electron micrographs of tibiae from 30 cases were examined. Bones of the 3 controls were ultrastructurally normal. Virions were observed in suspicious cases and positive cases ( +,++, + + + ) . In size, morphology, and ultrastructure they resembled avian type C oncoviruses. The observation provides pathogenic evidence for OP radiological diagnosis. The OP suspicious cases and positive cases ( +,++,+ + +) had ultrastructural changes characterized by osteocyte necrosis, enlarged bone lacunae, and vascular lesions etc. The correlation between radiology and electron microscopy provided ultrastructural evidence for OP radiological diagnosis.
出处
《中国农业科学》
CAS
CSCD
北大核心
2003年第10期1219-1222,T001,T002,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金(39770570)
