摘要
报告用酶联免疫吸附测定(ELISA)法测定人血清胰岛素抗体(IAb),批内及批间平均变异系数分别为8.3％、14.6％;阻断试验及交叉反应试验证明有较高的免疫学特异性。用本法调查了177名正常人血清中IAb,P/N值为0.9±0.27((?)±S),以>1.6((?)+2.5S)为阳性,阳性率为1.1％(2/177);接受胰岛素治疗的糖尿病(DM)组血清IAb阳性率为60％(27/45),与非胰岛素治疗的DM组、非DM组及正常对照组差异非常显著(P<0.01);非胰岛素治疗的DM组、肾病组及结缔组织疾病组阳性率分别为11.30％(9/80)、59％(3/51)、25％(5/20),与正常对照组比差异显著(P<0.01、0.05>P>0.01、P<0.01);结果提示ELISA法测IAb具临床特异性。本法简便,可为研究DM患者对胰岛素的免疫应答提供一个实用的工具。
An enzyme-linked immunosorbent assay(ELISA) for measurement of human insulin antibodies (IAb) is described.Intra-assay and inter-assay CV averaged 8.3% and 14.6%,respctively.Immunologic specificity of this ELISA was demonstrated by blocking test and cross reaction test.P/N of serum lAb in 177 healthy subjects,as determined by the method,was 0.910.27 (x±S).Positivity rate was 1.1%(2/177) in normal control group and 60% (27/45) in insulin treated diabetes group (p<0.01).They were 11.3% (9/80),5.9% (3/51) and 2.5% (5/20) in the diabetes group with no insulin treatment (P<0.01),renal disease group (0.05>p>0.01) and connective tissue disease group (P<0.01).The data showed that the ELISA method was clinically specific.ELISA is simple to perform,and it is a useful tool to study immune response to insulin in DM.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1989年第3期179-182,共4页
Medical Journal of Chinese People's Liberation Army
