摘要
本文用超排卵技术使雌性小鼠排卵,排卵前用药物或化学物处理后与雄性小鼠交配,以小鼠卵母细胞(MⅡ)及第一次分裂合子雌原核染色体非整倍体及结构畸变为指标,研究其诱变作用。结果表明,对照组小鼠卵母细胞(MⅡ)及第一次分裂合子均未发现有染色体非整倍体及结构畸变。处理组中,环磷酰胺和乙醇可诱发小鼠卵母细胞(MⅡ)及第一次分裂合于雌原核染色体非整倍体率增加,醋酸棉酚、氯氮平、苯妥英钠及安定则无明显影响。
In this research ICR female mice were stimulated to ovulate by the superovulation technique. Before ovulation, the female mice were treated with drugs and chemicals and then mated with male ones. The mutation-inducing effects of the drugs and chemicals were studied in terms of aneuploidy and chromosome aberrations in oocytes (MII) and female pronucleus of the first-cleavage zygotes.The results showed that no aneuploidy or chromosome aberration was found in the oocytes (MII) and first-cleavage zygotes in the mice of the control group. In the treated group, cyclophosphamide and ethanol induced an increased percentage of aneuploidy in the oocytes (MII) and female pronucleus of the first-cleavage zygotes, while gossypol acetate, clozapine, phenytoin and diazepam exerted no such influence.
出处
《生殖与避孕》
CAS
CSCD
北大核心
1992年第6期34-39,81,共6页
Reproduction and Contraception
关键词
药物
卵母细胞
染色体畸变
胚胎
Drug, Mouse, Oocyte, Zygote, Chromosome aberration, Chromosome nondisjunction
