摘要
目的 探讨糖尿病 (DM)患者血浆白细胞介素 18(IL 18)水平及外周血单个核细胞 (PBMCs)IL 18mRNA表达量变化。方法 选取 10名健康志愿者及 30例DM患者 (Ⅱ型糖尿病 15例 ,Ⅰ型糖尿病 15例 )。应用IL 18特异的酶联免疫吸附法(ELISA)测定IL 18血浆水平 ,同时采用半定量逆转录多聚酶链反应 (RT PCR)技术 ,分析PBMCsIL 18mRNA表达量。结果 Ⅱ型糖尿病患者血浆IL 18水平及PBMCsIL 18mRNA表达量与正常对照组差异无统计学意义 [(2 73.1± 99.4 6 )vs (2 4 8.86± 82 .6 8)and(0 .731± 0 .12 9)vs (0 .715± 0 .116 ) ,P >0 .0 5 ];Ⅰ型糖尿病患者血浆IL 18水平及PBMCsIL 18mRNA表达量较正常对照组增高 ,差异有显著统计学意义 [(36 0 .17± 12 5 .4 8)vs (2 4 8.86± 82 .6 8)and (0 .935± 0 .132 )vs (0 .715± 0 .116 ) ,P <0 .0 5 ]。结论 Ⅰ型糖尿病患者外周血IL 18的高表达 ,提示其可能介导本病发病过程 ;IL
Objective To investigate the expression of interleukin 18(IL-18) in patients with diabetes mellitus(DM).Methods Plasma level of IL-18 and the expression of IL-18 mRNA in peripheral blood mononuclear cells (PBMCs) were evaluated by enzyme-linked immunosorbent assay(ELISA) and reverse transcriptase polymerase chain reaction(RT-PCR) respectively in 10 healthy volunteers and 15 patients with type Ⅱ diabetes and 15 patients with typeⅠ diabetes. Results Plasma level of IL-18 and expression of IL-18 mRNA in PBMCs in patients with type Ⅱ diabetes were not different statistically when compared with those of healthy controls [(273.1 ± 99.46) vs (248.86 ± 82.68) and ( 0.731 ± 0.129) vs (0.715 ± 0.116), P > 0.05], but they were increased significantly in the patients with typeⅠ diabetes[(360.17 ± 125.48) vs (248.86 ± 82.68) and (0.935 ± 0.132) vs(0.715±0.116), P < 0.05]. Pearson correlation analysis showed that the levels of plasma IL-18 were not correlated with the levels of FBG. Conclusion Peripheral blood IL-18 may play a role in the initiation and progress of typeⅠdiabetes. but it may not be involved in type Ⅱ diabetes.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第6期464-466,共3页
Immunological Journal
