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牛肠激酶次特异性识别序列分析 被引量：3

Analysis of Sub-specific Cleavage Sites of Bovine Enterokinase
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摘要 在酶切融合蛋白时 ,除正常切割和非特异性切割以外 ,发现了牛肠激酶的一个可能的次特异性识别序列。分析了其产生的原因以及在基因工程研究中的意义。 Apart from normal specific cleavage and nonspecific cleavage, a possible sub-specific site was discovered in cleaving a fusion protein with enterokinase; the causes and the significance in the gene engineering were discussed.
作者 查向东 孔小卫 周立志
机构地区 安徽大学生命科学学院
出处 《安徽农业科学》 CAS 2003年第5期708-709,712,共3页 Journal of Anhui Agricultural Sciences
关键词 牛肠激酶 非特异性切割 次特异性切割 Bovine enterokinase,Non-specific cleavage,Sub-specific clevage site
分类号 Q78 [生物学—分子生物学]
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参考文献8

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同被引文献26

  • 1LeiFANG,Qi-MingSUN,Zi-ChunHUA.Expression of Recombinant Chinese Bovine Enterokinase Catalytic Subunit in P. pastoris and Its Purification and Characterization[J].Acta Biochimica et Biophysica Sinica,2004,36(7):513-517. 被引量：9
  • 2张向辉,谭树华,李泰明.肠激酶特点及其基因工程的研究进展[J].药物生物技术,2005,12(5):347-350. 被引量：9
  • 3顾玮彦,黄磊,徐志南,岑沛霖.利用his-tag纯化和复性基因工程产物肠激酶[J].食品与生物技术学报,2006,25(6):18-22. 被引量：2
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  • 6Song H W, Choi S I, Seong B L. Engineered recombinant enteropeptidase catalytic subunit: effect of N-terminal modification[J]. Arch Biochem Biophys, 2002,400 (1) : 1-6.
  • 7Lu D, Yuan X, Zheng X, et al. Bovine proenteropeptidase is activated by trypsin, and the specificity of enteropeptidase depends on the heavy chain[J]. Bio Chem, 1997,272 (50) :31293-31300.
  • 8Vozza L A,Wittwer L, Higgins D R,et al. Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris[J]. Nature Biotechnology, 1996,14= 77-81.
  • 9Collins-Racie L A,McColgan J M,Grant K L,et al. Production of reeombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA [J]. Nature Biotechnology, 1995, 13 : 982- 987.
  • 10Peng L S,Zhong X F,Ou J X,et al. High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris [J]. Journal of Biotechnology, 2004, 108:185-192.

引证文献3

二级引证文献14

安徽农业科学
2003年 第5期

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