摘要
采用Plackett-Burman设计对影响胰酶活力和提取率的13个因素进行筛选,有效筛选出主效因素(P<0.05):壳聚糖、CaCl2、胰腺与十二指肠配比和激活pH值。在此基础上,再运用均匀设计对以上4个显著因素的最佳水平范围进行研究,通过偏最小二乘法建立回归方程求解得到胰酶制备最适条件为壳聚糖用量0.17%、CaCl2用量0.06%、胰腺与十二指肠配比8.26:1、激活pH5.37。此条件下验证实测值胰蛋白酶、胰脂肪酶、胰淀粉酶活力和胰酶提取率分别为3408.71U/g、33.11kU/g、23.93kU/g和13.22%,与理论预测值的相对误差分别为7.15%、8.56%、10.63%、1.93%。Plackett-Burman设计和均匀设计相结合的方法在双激活剂胰酶壳聚糖絮凝法制备工艺中具有可行性。
With the aim of developing a method to prepare bovine trypsin by dual-factor activation and subsequent chitosan flocculation, Plackett-Burman design was used to evaluate the effects of 13 process conditions on the activity and extraction rate of trypsin to screen out 4 most significant ones （P〈0.05） including chtosan amount, ratio of bovine pancreas to duodenum and activation pH. Their optimal levels were optimized by the combined use of uniform design and partial least squares regression as follows： chtosan amount of 0.17%, CaCl2 amount of 0.06%, pancreas-duodenum ratio of 8.26：1, and activation pH 5.37, respectively. Under the optimized conditions, the activities of trypsin, lipase and amylopsin and the extraction rate of trypsin were observed to be 3408.71 U/g, 33.11 kU/g, 23.93 kU/g and 13.22%, respectively, which exhibited a relative error of 7.15%, 8.56%, 10.63% and 1.93% when compared with their predicted counterparts. Therefore, the combined use of Plackett-Burman design and uniform design is feasible to optimize the preparation of trypsin by dual-factor activation and subsequent chitosan flocculation.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第14期90-95,共6页
Food Science
基金
甘肃省农业生物技术研究开发项目(GNSW-2007-06)
