摘要
目的:研究补肾方对二乙基亚硝胺(DEN)诱发大鼠肝癌的预防作用。方法:以100ppmDEN饮水法诱发大鼠肝癌为模型,同时中药组予补肾方水煎剂灌胃,分别于诱癌12周末和18周末分批处死大鼠,观察大鼠肝脏病理形态学改变、肝组织γ-GT和AFP阳性表达以及血清丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)和白蛋白(Alb)含量变化。结果:中药组肝癌发生率较低;肝细胞增生灶和癌灶形成数目、面积较小;肝组织γ-GT阳性灶和AFP阳性细胞均明显比模型组少(P<0.001);血清ALT、ALP含量有不同程度降低,Alb含量较高。结论:补肾方对DEN诱发大鼠肝癌前病变和肝癌形成具有一定的抑制和延缓作用,但并不能完全阻断肝癌的形成。
Objective: To study the preventive effect of prescription of nourishing Shen on rat hepatocarcinoma induced by diethylnitrosamine (DEN) . Methods: Rats with hepatocarcinoma induced by 100ppm DEN were administered with the elixa-tion decoction of prescription of nourishing Shen at the same time. At the end of 12th week and 18th week, the rat were killed and the pathological changes of the liver, γ- GT positive focuses, AFP positive cells, as well as the alaninne aminotransferase (ALT), alkaline phosphatase (ALP) and albumin (Alb) in serum were observed. Results: In the group of prescription of nourishing Shen , the hepatocarcinoma incidence was lower than that in the model group ; the number and the area of hepato-cellular hyperplastic focuses and carcinoma focuses were little ; the γ-GT positive focuses and AFP positive cells were significantly little than those in the model group (P<0.001); serum ALT and ALP were lower than those in the model group at different levers , while Alb was higher.Conclusion: The prescription of nourishing Shen can inhibit and delay the development of rat liver precancerous lesion and hepatocarcinoma induced by DEN to a certain extent, but it can not block the production of hepatocarcinoma completely.
出处
《中西医结合肝病杂志》
CAS
2003年第5期278-280,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
关键词
补肾方
二乙基亚硝胺
大鼠
肝癌
药效学
病理形态学
TCM Science
Prescription of Nourishing Shen/Pharmacodynamics
Hepatocarcinoma
Diethylnitrosamine
Rat
