摘要
本文报道磁场对庆大霉素产生菌降红小单孢菌GB_5的孢子、发芽孢子和原生质体的磁致诱变效应。在所选用的九种磁场剂量处理后,三种状态的菌种的死亡率均在60％以上,其中原生质体的死亡率达96％以上,正变率12％以上,最高达49.1％,变异率在21％以上,摇瓶初筛获得抗生素产量提高10％以上的菌种占过筛菌株数的6.1％,其中产量提高30％以上的占1.5％.
The present study deals with the variation effected by magnetic fields of spores, sporoution spores and plasmid of Micromonospora purpurea—a gentamicin producing strain. The study showed that death rate of strains in 3 out of the nine cases of different magnetic field strengths was higher than 60%, the death rate of plasmid was higher than 96%, the positive mutation frequencies ranged from 12% to 49.1%, and the frequencies of variation were higher than 21%.
Through shake flask selection the production ability of 6.1% of the strains was found higher than that of the parent strains 6.1% by 10% and 1.5% by 30%.
关键词
庆大霉素
降红小单孢菌
磁场
Gentamicin
Micromonospora purpurea
Magnetic fields
Variation
