摘要
利用研制的显微分光光度和图像分析系统,对在组织工程可降解材料上三维培养的雪旺氏细胞,从生长形态、细胞特定蛋白表达量、细胞分化率、细胞突触网化度和DNA的受损情况进行分析,多方面地分析了雪旺氏细胞的生命活性指标,建立和提供了生物材料相容性研究的一些分析方法和技术手段。
The activity detection of schwann cell (SC) cultured on the bracket of tissue engineering material was performed using our multi channel micro-spectrophotometer system and micro-imaging analysis system- as well as the single cell gel electrophoresis analysis system. The DNA damage of the SC which was cultured on the glutaraldehyde crossed chtosan(GCC) filament of degradable tissue engineering material and the SC cultured on PGA filament material were analyzed- along with the analysis on the cell differentiation- and the expression of SC cells' special protein. The biological compatibility of the material can be evaluated according to the experiment results.
出处
《中国医学物理学杂志》
CSCD
2003年第4期222-223,226,共3页
Chinese Journal of Medical Physics
关键词
组织工程
细胞
活性检测
显微多道分光光度
单细胞凝胶电泳
tissue engineering
cell
activity detection
multi channel micro-spectrophotometry
single cell gel electrophoresis
