摘要
目的 :建立同时测定血浆中艾司唑仑、三唑仑和阿普唑仑浓度的方法。方法 :采用HPLC法 ,选用ZORBAXRP C18柱(15 0mm× 4 .6mm ,5 μm) ;甲醇 2 5mmol·L-1醋酸铵溶液 (5 7∶4 3)作流动相 ;检测波长为 2 30nm。结果 :本法对三唑仑、阿普唑仑和艾司唑仑的最低检测限浓度均为 4 μg·L-1;回收率接近 10 0 % ;日内、日间精密度 <5 % ;线性范围均为 2 0～ 10 0 0 μg·L-1。结论 :本法能同时测定血浆中三唑仑、阿普唑仑和艾司唑仑 ,此法重现性好 ,灵敏 ,可靠 ,可用于临床药物中毒监测。
OBJECTIVE To establish a method to determine simultaneously triazolam, alprazolam and estazolam in plasma by RP-HPLC.METHODS The reversed-phase C_ 18 column was used, the mobile phase consisted of methanol-25 mmol·L -1 NH_4Ac(57∶43),the absorbance of eluent was monitored at 230 nm.RESULTS The detection limit of this method was 4 μg·L -1 for triazolam, alprazolam and estazolam, the method recovery approached 100%, RSD was less than 5%, and their linearity range was 20～1000 μg·L -1 in plasma.CONCLUSIONS This sensitive and selective method can be used for determining simultaneously triazolam, alprazolam and estazolam in plasma and for therapeutic drug monitoring. This method has good recovery and reliability.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2003年第10期599-601,共3页
Chinese Journal of Hospital Pharmacy
