摘要
目的 在多器官功能衰竭 (MOF)时肠黏膜肥大细胞 (IMMC)呈过度活化 ,P物质 (SP)是广泛参与机体生理和病理调节的胃肠多肽。探讨SP在MOF时对IMMC活性的调节作用。方法 酵母多糖腹腔注射法制作大鼠MOF模型 ,经尾静脉输入SP (剂量为 2 0nmol/kg体重和 0 .2nmol/kg体重 ) ,观察动物肠、肝、肾、肺等器官的病理改变及ALT、Cr、血氧分压 (PO2 )等指标变化 ,测定动物外周血和小肠组织组胺及肿瘤坏死因子 α(TNF α) ,透射电镜观察IMMC超微结构。结果 与MOF对照组相比 ,SP组大鼠重要器官的炎症病变明显加重 ,ALT升高 4 0 %～ 5 0 %、Cr升高 4 5 %～5 0 % ,PO2 降低约 4 0 % (P <0 .0 1)。SP两剂量组间无显著差异 (P >0 .0 5 )。SP组MOF大鼠外周血组胺无明显变化 (P >0 .0 5 ) ,小肠组织组胺则降低约 4 4 % (P <0 .0 1) ,SP两剂量组间小肠组织组胺无显著差异 (P >0 .0 5 )。SP组大鼠外周血TNF α无明显变化 (P >0 .0 5 ) ,小肠组织TNF α升高近 5 0 % (P<0 .0 1) ,SP两剂量组间小肠组织组胺无显著差异 (P >0 .0 5 )。SP组大鼠IMMC胞质颗粒数量进一步减少 ,表现为颗粒包膜相互融合形成的空泡更大、更多 ,SP两剂量组间IMMC超微结构差异不明显。结论 SP促进MOF时IMMC脱颗粒 ,降低其稳定性 ,分泌组胺和TNF α。
Objective Multiple organ failure (MOF) has been regarded as a continuous, uncontrolled inflammatory response. Intestinal mucosal mast cells (IMMC) may be involved in MOF. Substance P (SP), one of gut peptides, is an important regulator in the neuro-endocrine-immune network. However, the effects of SP on IMMC, especially in the case of MOF, remain unclear. This study was aimed to investigate the effects of SP on IMMC in the development of MOF. Methods The rat model of MOF was established by injecting zymosan. After thirty minutes of the injection, SP was given through tail veins at the dose of 20 nmol/kg weight and 0.2 nmol/kg weight. The concentrations of histamine and tumor necrosis facfor-α(TNF-α) in plasma and intestine tissues were measured. The pathological alterations of essential organs including intestine, liver, kidney and lung were examined under light microscope. Their corresponding functions were reflected with ALT, Cr and PO 2. The ultrastructure of the IMMC was also observed under a transmission electronic microscope. Results Compared with the controlled rats, those injected with SP showed much more serious inflammatory response under light microscope. Both ALT and Cr increased by about 50%, but PO 2 decreased by about 40%. Histamine level in the intestinal tissue of the rats treated with SP remarkably decreased, whereas the plasma histamine level did not show any significant changes. The level of TNF-α was higher in the intestinal tissue of the rats treated with SP but no change in plasma, and the degranulation of IMMC under transmission electronic microscope was more obvious.Conclusions SP may trigger MOF through acting on IMMC which may release inflammatory mediators such as histamine and TNF-α.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2003年第5期271-274,共4页
Chinese Journal of Digestion
基金
国家杰出青年科学基金 ( 3 972 5 0 12 )
关键词
P物质
多器官功能衰竭
肠黏膜肥大细胞活性
调节
Intestinal mucosal mast cells
Multiple organ failure
Substance P
Histamine
Tumor necrosis factor-α
