摘要
背景:已知胃肠多肽生长抑素(SST)和血管活性肠肽(VIP)可调节肠黏膜肥大细胞(IMMC)的生物学活性。多数胃肠多肽对IMMC活性的调节作用可能与其受体途径有关。目的:探讨大鼠IMMC上是否有SST受体(SSTR)和VIP受体(VIPR)mRNA表达,确定SST和VIP调节IMMC活性的分子机制。方法:采用胶原酶消化法分离大鼠小肠IMMC,Percoll不连续密度梯度离心法纯化,逆转录聚合酶链反应(RT鄄PCR)观察IMMC上SSTR鄄1、SSTR鄄3和VIPR鄄1、VIPR鄄2mRNA的表达。结果:大鼠IMMC上有SSTR鄄1和VIPR鄄2mRNA表达,其电泳条带分别位于1183bp和494bp,但无SSTR鄄3和VIPR鄄1mRNA表达。结论:大鼠IMMC可表达SSTR鄄1和VIPR鄄2基因,SST和VIP对IMMC活性的调节作用可能是通过其相应受体SSTR鄄1和VIPR鄄2途径实现的。
Background: It has been proven that the biological activity of intestinal mucosal mast cells (IMMC) can be regulated by gut polypeptides as somatostatin (SST) and vasoactive intestinal peptide (VIP). Most gut polypeptides have their regulating roles on IMMC activity through the pathways of their receptors. Aims: To investigate the mRNA expression of the receptors for SST (SSTR) and VIP (VIPR) on IMMC in rats, and to determine the molecular mechanisms of the regulating roles of SST and VIP on IMMC activity. Methods: IMMC were isolated and purified from the small bowel of rats by collagenase digestion and Percoll discontinuous density gradient centrifugation, respectively, then the mRNA expression of SSTR-1, SSTR-3, VIPR-1 and VIPR-2 on IMMC were observed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: The rats' IMMC apparently expressed the mRNA of SSTR-1 and VIPR-2, the products were 1 183 bp and 494 bp, respectively, while the mRNA expression of SSTR-3 and VIPR-1 could not be detected. Conclusions: IMMC can express SSTR-1 and VIPR-2 genes. SST and VIP may regulate the activity of IMMC through SSTR-1 and VIPR-2 pathways.
出处
《胃肠病学》
2004年第3期161-163,共3页
Chinese Journal of Gastroenterology
基金
国家杰出青年科学基金(No.39725012)资助
