摘要
目的 探讨67ku层粘连蛋白受体(67LR)基因表达与子宫内膜癌的关系。方法 应用免疫组化SABC法检测1990～2001年收治的44例子宫内膜癌、15例子宫内膜增殖症、47例正常子宫内膜组织中67LR的表达;采用荧光定量逆转录聚合酶链反应(FQ-RT-PCR)方法定量检测2000～2001年收治的24例子宫内膜癌、16例子宫内膜增殖症、29例正常子宫内膜新鲜组织67LRmRNA水平。结果 67LR在子宫内膜癌、子宫内膜增殖症的表达较正常子宫内膜均明显增高(P<0.001),但前两组的表达差异无显著性(P>0.05)。高、中分化子宫内膜腺癌67LR表达较低分化者为高(P<0.05);但高、中、低分化组病例67LR mRNA水平差异无显著性(P>0.05)。67LR mRNA水平与免疫组化蛋白表达无明显相关(P>0.05)。结论 67LR蛋白过度表达可能与子宫内膜癌的发生、发展有关。67LR mRNA水平与蛋白表达水平无相关。
Objective To explore the expression of 67ku laminin receptor(67LR) and its correlation with endometrial carcinoma. Methods Immunohistochemistry was used to determine the expression of 67LR in 44 cases of endometrial carcinoma, 15 of endometrial hyperplasia,47 of normal endometrium. The FQ-RT-PCR method was used to measure the expression of 67LR mRNA in 24 cases of endometrial carcinoma, 16 of endometrial hyperplasia,and 29 of normai endometrium. Results 67LR showed notably increased expression in endometrial carcinoma and hyperplasia, compared with normai endometrium( P <0.001), while there was no significant difference berween the former two groups ( P >0.05). The ex-pression of 67LR in carcinoma of grade 1-2 was strikingly higher than those in grade 3( P < 0.05). There was no significant difference in the Ievel of 67LR mRNA among the three groups ( P > 0.05). The Ievel of 67LR mRNA had no correlation with the 67LR protein( P >0.05).Condusion The exaggerated expression of 67LR in endometriummay pky a pathologic role in endometrial carcinoma. The Ievel of 67LR mRNA has no relationship with its protein expression.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2003年第10期596-598,共3页
Chinese Journal of Practical Gynecology and Obstetrics
关键词
层粘连蛋白受体
基因表达
子宫内膜癌
免疫组化
检测
Laminin receptor Endcrnetrial carcinoma Fluorogenic quantitative-RT-PCR Immunohistochemistry
