摘要
采用荧光定量 RT- PCR法检测 36例不同类型白血病患者的多药耐药基因 (mdr1 m RNA)表达 ,同时检测 15例骨髓或外周血正常患者 ,以做对照。结果对照组均为阴性表达 ,初治患者组 m dr1 阳性基因拷贝数平均为 2 .7× 10 3拷贝 / μg RNA;复发难治患者组为 1.0× 10 4拷贝 / μg RNA(P<0 .0 5 )。表达阴性 m dr1 与 mdr1 阳性患者的完全缓解 (CR)率分别为 82 %和 2 1% (P<0 .0 5 )。提示荧光定量 RT- PCR检测 mdr1 基因表达结果用绝对拷贝数表示 ,其定量准确、可靠。临床急性白血病化疗耐药的发生主要与 mdr1 阳性高表达有关。
The expression of multi-drug resistance gene(mdr 1 mRNA)was measured in 36 patients with different type of acute leukemia and in 15 normal controls by fluorogenic probe quantitative RT-PCR(FQ-RT- PCR). Result showed that the mdr 1 mRNA was neg ative in normal controls. The average level of the mdr 1 gene express in untreated group was 2.7×10 3 copies/μgRNA,while that of clinical resistance group was 1.0×10 4 copies/μgRNA(P<0.05).The CR rate differed significantly between mdr 1 positive group(21%)and negative group(82%)(P<0.05).It suggests that FQ-RT-PCR is an acurate quantitative method in detecting the mdr 1 gene expression,a high level of mdr 1 expression correlated closely with drug resistance in clinical leukemia chemotherapy.
出处
《山东医药》
CAS
北大核心
2001年第5期3-4,共2页
Shandong Medical Journal
