摘要
由于H9N2亚型禽流感对我国的养鸡业已造成了很大的损失,因而在我国许多养鸡场不得不使用H9N2亚型禽流感灭活苗[1],但由于灭活苗的使用,而增加了H9N2亚型禽流感的监测难度.因此,建立一种能区别自然感染鸡和疫苗免疫鸡的鉴别诊断方法已被提到日程上来.
The nostructural protein (NS1) encoded by gene 8 of the Influenza virus is present in cells infected with Influenza virus. In this study, NS1 protein gene of the Chicken influenza virus A/chicken/Beijing/2/97 (H9N2) strain was amplified by PCR. The fragment contains EcoR Ⅰ and Xho Ⅰ restriction enzyme sites at the ends. The amplified product was cloned into the expression vector pET-30(c). Recombinant plasmid pET/NS1 was transformed into E.coli BL21(DE3) competent cells and induced with 0.4 mmol/L IPTG, the target protein was produced, the molecular weight of the expressed protein was 30 kDa as expected. Western-blot test indicated that the expressed protein can react with the NS1 monoclonal antibody of the influenza virus. This study laid an important foundation for H9N2 subtype avian influenza surveillance in China.
出处
《中国病毒学》
CSCD
2003年第5期503-505,共3页
Virologica Sinica
