摘要
应用微卫星标记技术和可溶性蛋白质含量测定 ,对水稻品种粤香占及其由高压引起的变异材料粤压 1、2、3、4号进行分子生物学研究。结果表明 ,在选取的 88对微卫星引物中有 11对表现多态性 ,变异材料与原种的多态性频率介于 3 .4%～11.3 % ,多态性标记随机地分布于水稻染色体上 ,形态上与原种越相近的材料多态性位点数越少。此外 ,每一变异材料叶片可溶性蛋白质含量互不相同 。
Rice variety Yuexiangzhan and its mutants induced by high pressure, were studied by using microsatellite marker and soluble protein content analysi s. Results indicated that 11 of the 88 microsatellite primer pairs showed polymorphism. The polymorphic frequency was 3.4%-11.3% between mutants and Yuexiangzhan. The polymorphic markers were randomly located on rice chromosomes. The more similar the plant type of the mutants looked like their original variety(Yuexiangzhan), the less polymorphic loci were obtained. In addition, content of soluble protein in leaves of mutant was significantly lower than that in the original variety.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2003年第4期373-375,共3页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目 ( 30 0 70 4 2 6 )
广东省自然科学基金资助项目 ( 990 981 )
