摘要
以青菜带柄的子叶为外植体 ,在MS培养基上附加 5mg L 6 BA、0 5mg LNAA和 5mg LAgNO3 ,能使再生频率明显提高。无菌苗和处理中的外植体经过 1～ 2 5Gy的γ射线低剂量辐照处理后 ,都能促进不定芽的再生 ,1 0Gy以上再生频率急剧降低。在共培养期经 1～ 7 5Gy的低剂量辐照后 ,gus基因瞬时表达频率提高 。
Cotyledons with petiole were cultured on MS basic medium containing 5mg/L BA, 0 5mg/L NAA, 5mg/L AgNO 3, and produced adventitious buds with higher frequency. Induction frequency of adventitious buds could be increased by irradiation treatment of γ rays with the lower dose of 1～2 5Gy to seedlings and explants in culture, but decreased dramatically when the dose was higher than 10 Gy. Irradiation treatment in co culture with lower dose of 1～7 5 Gy also promote transient expression frequency of gus gene, while irradiation treatment in pre culture did not show obvious effect on transient expression of gus gene.
出处
《核农学报》
CAS
CSCD
北大核心
2003年第5期336-339,共4页
Journal of Nuclear Agricultural Sciences
基金
国家科技部九五重点科技攻关 ( 96 0 15 0 1)
合肥市政府专项资助项目 (I2 0 10 6)
