摘要
目的 研究静脉注射人内皮型一氧化氮合酶 (eNOS)基因对果糖诱导高血压大鼠的降压作用及其对胰岛素抵抗的影响。方法 通过给雄性Sprague Dawley大鼠饮用 10 %的果糖水诱导制成高血压动物模型 ,2周后经静脉注入含人eNOScDNA的pcDNA·eNOS重组质粒。每周测血压 2次 ,并于注入eNOS重组质粒后 2周与对照组同时取血 ,测定血糖、血脂及血胰岛素水平 ,并记录 2 4h尿量。结果 一次静脉注射含人eNOS基因的重组质粒后第 3天 ,大鼠升高的收缩压明显下降至 ( 12 2 80± 0 2 )mmHg( 1mmHg =0 133kPa) ,最大降压效应在导入基因后第 14天 ,血压下降至 ( 12 0 30± 1 6 )mmHg并持续 3周以上 ,而对照组血压 [( 12 5 80± 0 4 )mmHg]无变化 (P <0 0 0 1)。实验组在血压下降的同时 ,血胰岛素水平也明显降低。结论 人eNOS基因的导入可能有助于降低血压 ,并改善高血压伴胰岛素抵抗 ,提高患者的胰岛素敏感性。
Objective To study the effect of gene transfer of human endothelial nitric oxide synthase(eNOS) on the blood pressure and insulin resistance in fructose-induced hypertensive rats(FHR). Methods Male Sprague-Dawley rats were fed with 10% fructose-water to develop hypertensive models. Then recombinant plasmid containing human eNOS cDNA(pcDNA·eNOS) was injected into FHR intravenously. The caudal arterial pressure was measured twice a week. Expression of eNOS gene in the rats was measured by Western blot in the second week after injection. Blood samples were collected for determination of the levels of blood glucose and insulin. Results A single injection of pcDNA·eNOS caused a significant reduction of systolic blood pressure for 3 weeks and also plasma insulin and glucose levels in FHR compared with control group from 3 days to 3 weeks after injection(P<0.01).Conclusion Somatic delivery of the human eNOS gene induces a reduction of high blood pressure and improves the insulin sensitivity. It suggests that eNOS gene may be used to treat hypertension.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2003年第4期289-292,共4页
Chinese Journal of Cardiology
基金
武汉市科委资助项目 ( 9970 0 5 135G)
