摘要
采用流式细胞分析术(FCM)检测15例正常子宫内膜组织与28例子宫内膜癌组织的DNA含量与细胞周期分布,并由此推算出DNA指数(DI)和S期细胞比率(SPF),结合雌、孕激素受体检测结果,从分子生物学角度对子宫内膜癌的生物学特性进行了研究。15例正常子宫内膜组织均为二倍体,其DI为0.93～1.07,而28例子宫内膜癌中二倍体有18例(64％),异倍体出现率与组织分化程度及受体状况密切相关(P均<0.05),SPF亦与分化程度相关。
The DNA contents in cells from 15 histopathologically normal endometria and 28 endometrial carcinoma was measured by flow cytometry (FCM). In the normal cases, only single DNA peak was found, all in the diploid region and the range of DNA index values (0.93-1.07) was used for defining the limits of DNA for diploid tumor cases. Aneuploid cell population was found in 36% of malignant endometria and in the remained cases, the FCM finding showed no differences as compared with those of benign tissue. Ploidy aberations were correlated to histologic tumor grades. Aneuploidy was more common (100%) in poorly differentiated tumors than in the highly or moderately differentiated (15.4%) (P<0.05). Receptor status was significantly correlated with ploidy as diploid tnmors were more aften receptor positive (P<0.05), but it was not correlated to FIGO stage, depth of myometriali invasion and spread to vessel. In normal endometria, the fraction of cells with DNA content corresponding to s-phase(s-fraction) was 14% in average. In well and moderately differentiated diploid tumors the s-fraction was about the same (13.7% vs 16%), but in the poorly differentiated it was significantly higher (37%):
出处
《上海医学》
CAS
CSCD
北大核心
1992年第11期639-642,共4页
Shanghai Medical Journal
关键词
子宫内膜癌
子宫肿瘤
FCM
Endometrial carcinoma
Flow cytometry
DNA index
s-fraction
