摘要
目的 从HIV AIDS患者应用微量全血法分离中国HIV 1毒株 ,研究HIV 1的生物学特性与HIV AIDS疾病进展相关性。方法 建立微量全血法 ,从HIV AIDS全血标本中分离 17株HIV 1病毒分离株 ;检测这 17株病毒分离株嗜性和复制动力。结果 从 2 6例HIV AIDS病例中分离出HIV 1病毒 ,分离率为 6 5 .4 % (17 2 6 ) ,其中 17例HIV 1感染者的病毒分离率为 5 2 .9% (9 17) ,均为巨噬细胞嗜性 (M嗜性 ,NSI) ;9例AIDS患者的HIV 1病毒分离率为 88.9% (8 9) ,其中 7株为T细胞嗜性 (T嗜性 ,SI) ,1株为巨噬细胞嗜性。通过检测P2 4抗原确定 17株HIV 1病毒分离株的复制动力。在分离到的 17株HIV 1中 ,SI型病毒分离株与AIDS组显著相关 (P <0 .0 5 ) ;AIDS期的病毒分离株的复制动力明显高于HIV感染期 (P <0 .0 5 )。结论 微量全血法可用于病毒分离。 17株分离株的HIV 1复制动力与CD4 + T淋巴细胞计数呈线性负相关 ,与病毒载量呈正相关。
Objective To isolate HIV-1 stains from HIV/AIDS with microseparation of the whole blood specimens, and to identify the association between HIV-1 biological features and HIV/AIDS disease progression. Methods Seventeen HIV-1 isolates were collected from 26 HIV/AIDS specimens and cell tropism of HIV-1 isolates were tested with MT-2 and microphage. Viral antigen P24 of the culture supernatants was used as a marker of the viral replication. Results Seventeen HIV-1 isolates were collected from 26 HIV/AIDS, and isolation rate could reached 65.4%, of which the isolation rate from HIV-1 infected specimen was 52.9%, isolates were M tropism; and that from AIDS patients were 88.9%, seven isolates were T tropism and one isolates was M tropism. Replication dynamics of seventeen HIV-1 isolates was detected by P24 antigen capture assay. Of the isolates, correlation was significantly different between SI isolates and AIDS group ( P <0.05). Replication dynamics of AIDS isolates were significantly higher than that of HIV-1 infected isolates ( P <0.05). Conclusions Small volume of blood is needed for HIV isolation. Replication dynamics of seventeen isolates were linearity correlation with CD4 + lymphocyte counts and viral load.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2003年第9期703-706,共4页
Chinese Journal of Microbiology and Immunology
基金
国家重大基础研究规划 973资助项目 (G19990 5 410 7)
