摘要
目的 :观察内皮素 (ET)对大鼠心肌细胞牛磺酸转运的影响。方法 :在培养的乳鼠心肌细胞上 ,应用同位素标记的方法测定心肌细胞牛磺酸转运。结果 :内皮素呈浓度依赖性 ( 10 -10 ～ 10 -8mol/L)抑制心肌细胞牛磺酸转运 (与对照组比较分别下降了 13 %,3 8%和 71%,P <0 0 1)。蛋白激酶C(PKC)的抑制剂H7,ET -A型受体阻断剂BQ12 3及用佛波脂 (PMA)预处理均可拮抗ET的抑制作用 ,使牛磺酸转运较ET组分别增加 2 2 1%,2 5 0 %和 2 82 %(P <0 0 1)。结论 :ET通过其A型受体活化PKC抑制心肌细胞牛磺酸转运。
AIM: To investigate the effect of endothelin(ET) on taurine transportation in rat cardiac myocytes in vitro. METHODS: In cultured cardiac myocytes of neonatal rats, taurine transportation velocity was measured by radio-ligand method. RESULTS: ET(10 -10 -10 -8 mol/L) could inhibit taurine transportation in a dose-dependent manner.10 -10 ,10 -9 and 10 -8 mol/L of ET significantly decreased taurine transpotation by 13%, 38% and 71%, respectively (P<0.01), compared with control group. H 7,BQ123 and Pre-PMA can reverse the inhibition of ET on taurine transportation dramatically(P<0.01). CONCLUSION: The binding of ET and ET-A receptor might activate protein kinase C,which inhibits taurine transportation in cultured myocytes of rats.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第2期116-119,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助 (No 3 973 0 2 2 0 )
