摘要
目的 检测多药耐药基因 1(multidrugresistance 1,mdr1)和多药耐药相关蛋白 1(multidrugresistance associatedpro tein ,MRP1)基因在不同发育阶段豚鼠耳蜗外侧壁细胞中的表达情况 ,并讨论其在血迷路屏障中的防护作用。方法 利用原位杂交技术检测mdr1/MRP1mRNA的表达 ,采用MPLAS 5 0 0计算机图像处理系统进行图像分析。结果 (1)豚鼠耳蜗外侧壁中mdr1mRNA主要在微血管内皮细胞中表达 ,MRP1mRNA主要在边缘细胞中表达。 (2 )mdr1/MRP1mRNA在不同发育阶段豚鼠耳蜗外侧壁中的表达强度存在差异 :3周龄与 4周龄豚鼠间mdr1mRNA表达强度差异无显著意义 (P >0 .0 5 ) ;但两者较 1～ 2周龄豚鼠mdr1mRNA表达强 ,并有显著性意义 (P <0 .0 1) ;MRP1mRNA在 1～ 2周龄耳蜗外侧壁中弱表达 ,与 3周龄和 4周龄间差异有显著意义 (P <0 .0 1)。 (3)在 1～ 2周龄豚鼠耳蜗外侧壁中主要是mdr1mRNA表达 ,而MRP1mRNA表达较少 ,两者间差异有显著意义 (P <0 .0 1) ,而 3周龄和 4周龄豚鼠耳蜗外侧壁中两者表达差异无显著意义 (P >0 .0 5 )。结论 mdr1/MRP1mRNA存在于正常耳蜗外侧壁中 ,随着耳蜗外侧壁的发育其表达强度不同 ,并且两者的表达存在不同步性。
Objective To examine the expression and the significance of mdr1 /MRP1mRNA in guinea pig cochlea lateral wall at different developmental stages.Methods Thirty white guinea pigs with red eye including three developmental stages were used in this study.After cardiacally perfused,the auditory vesicle were taken out and cochlea lateral wall were quickly dissected under anatomic microscope,then immersed in the 4% citromint.For in situ hybridization,tissues were cryostat sectioned(10μm).Results mdr1mRNA were detected mainly in the endothelial cells,in contrast to,MRP1mRNA mainly in the border cells.The both were clearly found in cochlea lateral wall of the guinea pigs 4 weeks old or above old.MRP1mRNA were hardly found in the guiaea pigs 1～2 weeks old.Conclusion Firstly,mdr1/MRP1mRNA were found in the normal cochlea lateral wall cells.The orientation and the reaction degree are different with the development of guinea pigs cochlea lateral wall.Secondly,the both are not expressed synchronously during the development of guinea pigs cochlea lateral wall.
出处
《重庆医学》
CAS
CSCD
2003年第11期1450-1453,共4页
Chongqing medicine
