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Fy(a-b-)的基因定型 被引量:1

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摘要 目的 介绍新的Duffy沉默基因 (FY)检测方法 ;探讨该方法在输血学和人类学中的意义。 方法 首先采用文献介绍的聚合酶链反应 限制性酶切片短长度多态性 (PCR RFLP)方法[6] 检测样品的Duffy基因型 ,然后对可能含有FY基因的基因型个体用笔者设计的PCR RFLP法检测其是否存在FY基因。结果 在所有可能含有FY基因的辽宁汉族人基因型中未发现FY基因。结论 中国辽宁汉族人群中FY频率极低或辽宁汉族人群与黑人的FY等位基因有不同的遗传背景 ;用PCR RFLP法检测Duffy血型 ,可直接判定基因型而不是表现型 ,避免血清学检测引起的误差。
出处 《中国输血杂志》 CAS CSCD 2003年第5期314-315,共2页 Chinese Journal of Blood Transfusion
基金 辽宁省自然科学基金资助项目 (No .972 2 3 4)
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