摘要
目的 寻找前列腺癌相关基因以用于前列腺癌的诊断和治疗。 方法 应用含有4 36 6条人类全长基因cDNA基因表达谱芯片 ,研究一组前列腺癌和正常前列腺组织样本的基因表达谱 ,找出研究前列腺癌组织和正常前列腺组织中差异表达的基因。 结果 前列腺癌与正常前列腺组织有差异表达基因 2 87条 ,其中未知基因 16 5条 ,已知基因 12 2条 ,从已知基因中筛选出有显著差异表达的基因 5 6条 ,其中上调基因 2 0条 ,下调基因 36条。 结论 运用cDNA微矩阵基因芯片对基因表达谱进行分析 ,能有效筛查出新的前列腺癌相关基因。
Objective To investigate the genes differentially expressed in prostate cancer and to determine some prostate cancer associated genes for diagnosis and treatment prostate cancer. Methods cDNA microarray chips containing 4 366 human genes in full length were used to investigate the differential gene expression patterns of samples from human prostatie cancers and normal prostate tissues. Results A total of 287 genes differentially expressed with prostatie cancers and normal prostate were screened out,comprising 165 unknown genes and 122 known genes.Among the known genes we further identified 56 genes which were markedly differentially expressed (20 up-regulated and 36 down-regulated genes). Conclusions cDNA microarray chips for analysis of genes expression patterns is a powerful method for identification of novel prostatie cancer associated genes.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2003年第11期757-759,共3页
Chinese Journal of Urology
基金
国家自然科学基金资助项目 ( 3 9760 0 72 )
