摘要
目的:建立利肝片中猪去氧胆酸(HDCA)及鹅去氧胆酸(CDCA)的含量测定方法。方法:在硅胶G板上以环已烷-醋酸乙酯-醋酸-甲醇(20:25:2:3)展开,以薄层荧光扫描法,激发波长为366 nm,测定利肝片中HDCA及CDCA的含量。结果:平均回收率分别为HDCA:99.9％,RSD=1.9％;CDCA:100.9％,RSD=2.8％。结论:该方法灵敏,准确,适于作为该制剂的质控标准。
OBJECTIVE To establish a method for determination of hyodeoxycholic acid (HDCA) and chenodeoxycholic acid (CDCA) in Ligan tablets. METHODS The separation of HDCA and CDCA was accomplished on the silica gel plate by development using cyclohexane-ethyl acetate-acetic acid -methanol (20:25:2:3) . The contents of HDCA and CDCA was determination by fluorescent TLC scanning with λex = 366 nm (No. 2 filter). RESULTS The method had a good linearity. The average recovery of HDCA was 99.9 % with RSD of 1.88 % . The average recovery of CDCA was 100.9 % with RSD of 2.78 % . CONCLUSIONS The method is sensitive and accurate for the quality control of Ligan tablets.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2003年第11期651-652,共2页
Chinese Journal of Hospital Pharmacy
关键词
利肝片
薄层荧光扫描
猪去氧胆酸
鹅去氧胆酸
Ligan tablets
TLC fluorescence scanning
hyodeoxycholic acid
chenodeoxycholic acid
