摘要
根据隐孢子虫 18S r DNA序列 ,设计出隐孢子虫属和鼠隐孢子虫种的特异性引物 ,进行 PCR和 Nested PCR反应 ,先后分别扩增出 1条 5 4 0 bp和 1条 2 5 0 bp的条带。研究表明 ,Nested PCR具有高度的特异性和敏感性。应用Nested PCR可检测 1～ 10个鼠隐孢子虫卵囊 ,其敏感性是饱和蔗糖漂浮法的 10 5倍以上。
Two sets of primers special to Cryptosporidium and Cryptosporidium muris were designed and synthesized respectively based on the sequence of 18 S rDNA.A fragment of 540 bp and 250 bp was amplified by polymerase chain reaction(PCR) and nested PCR respectively using DNA of Cryptosporidium muris.It was found that the nested PCR showed the highest specificity and sensitivity when compared with conventional PCR,sheather′s floating,auramine phenol and acid fast staining.Using the nested PCR,one to ten oocysts in samples could be detected with the sensitivity of about 10 5 times higher than that of sheather′s floating.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2003年第6期570-572,共3页
Chinese Journal of Veterinary Science
基金
安徽省自然科学基金资助项目 (0 10 412 0 4)
