摘要
为建立一套行之有效的隐孢子虫灭活效果评价方法,在构建鸡源隐孢子虫C. baileyi动物模型的基础上,选用3种检测方法(抗酸染色、饱和蔗糖悬浮镜检和荧光检测),对鸡源隐孢子虫进行镜检、数量统计,并比较3种方法的优劣及适用范围。结果显示:荧光染色法具有最佳的检测效率(检出数量为改良抗酸染色法,饱和蔗糖悬浮镜检法的2.1和2.6倍;检测时间为120 min),并能够进行隐孢子虫的活性鉴定,可以作为饮用水消毒剂灭活隐孢子虫的评定方法,是一种高效、简便、较为廉价,并具有较大发展前景的检测方法。
In order to construct an efficient method for evaluating disinfection effect of Cryptosporidium, based on establishing a Cryptosporidium bailey infective model in chick, three techniques were used for examining the quantity of C. bailey oocyst： acid-fast staining, saturated sucrose flotation inspection, fluorescence staining detection. The results show that fluorescence staining detection has the best measurement efficiency, the number of measured oocyst is 2. 1 and 2.6 times of acid-fast staining and saturated-sucrose flotation inspection, while the examination process takes 120 minutes, furthermore, fluorescence staining detection can identify the viable oocyst and may be a standard method for evaluating disinfection effect in drinking water contaminated by Cryptosporidium. Fluorescence staining detection is a highly efficient, simple and cheap examination method, and has extensive development prospects.
出处
《环境工程学报》
CAS
CSCD
北大核心
2009年第1期47-51,共5页
Chinese Journal of Environmental Engineering
基金
国家"863"高技术研究发展计划项目(2006AAZ309)
关键词
隐孢子虫
荧光染色
Cryptosporidium
fluorescence staining detection
