摘要
目的氧自由基和血管紧张素Ⅱ(AngⅡ)过多是造成心肌损害的重要机制,为有效保护心肌,为心肌损害治疗开辟一个新途径,本文研究参麦注射液(SMI)、生长激素(GH)、硫酸锌(ZnSO4)、维生素C(VitC)对过氧化氢(H2O2)或AngⅡ损伤下降的心肌细胞存活力(CMV)的影响。方法 用新生大鼠心肌细胞加入H2O2(200 μmol/L,500μmol/L)或AngⅡ(10-6mol/L,10-7mol/L)使CMV下降作为对照组(共4组);在加入H2O2或AngⅡ的同时加入SMI(5 ml/L和10 ml/L),VitC(50 mg/L),ZnSO4(15μmol/L和60 μmol/L)或GH(250 μmol/L和500μmol/L)作为保护组。检测各组在加药24 h后的CMV增高率。结果SMI,GH,ZnSO4和VitC均可显著提高因H2O2损伤而下降的CMV增高率。以SMI 10 ml/L的效果最好;其次为ZnSO4 60 μmol/L;再其次为SMI 5 ml/L,VitC 50 mg/L;最差为GH 500 μmol/L,GH 250 μmol/L和ZnSO4 15 μmol/L,其中SMI 5 ml/L和VitC 50 mg/L间差异无显著性,后3者间差异也无显著性。SMI和GH可显著提高AngⅡ所致的CMV下降,以SMI 10 ml/L效果最好;其余依次为SMI 5 ml/L,GH 500 μmol/L和GH 250μmol/L,但后2者间差异无显著性(P>0.05)。结论SMI和GH对因H2O2或AngⅡ损伤下降的CMV有显著提高作用。
Objective The increase of oxygen-derived free radicals (OFR) or angiotensin Ⅱ(Ang Ⅱ ) in blood is one of the important causative agents of myocardium lesions. To develop a remedy for treatment of myocardium lesions induced by OFR or Ang Ⅱ , this study aims at exploring the protective effects of shenmai injection (SMI), growth hormone (GH), zinc sulfate (ZnSO4) and vitamin C (Vit C) on the cardiac myocytes injured by hydrogen peroxide (H2O2) or Ang Ⅱ. Methods H2O2 with the concentrations of 250 and 500 μmol/L or Ang Ⅱ with the concentrations of 10 6 and 10 7 mol/L were added respectively to the cardiac myocytes of neonatal rats so as to reduce the cardiac myocytes viability ( CMV). The injured cardica myocytes were administrated respectively with 5 ml/L and 10 ml/L (concentration) of SMI, 50 mg/L of Vit C, 15μmol/L and 60 μmol/L of ZnSO4, and 250 μmol/L and 500 μmol/L of GH. The increasing rate of CMV was measured at 24 h after medication. Results SMI, GH, ZnSO4 and VitC could all significantly elevate the increasing rate of CMV which had been decreased by H2O2 injury. The best effect was made by the SMI 10 ml/L group, followed in turn the ZnSO4 60 μmol/L, SMI 5 ml/L, Vit C 50 mg/L, GH 500 μmol/L, 15 μmol/L ZnSO4 and 250 μmol/L GH groups. There was no significant difference between the SMI 5 ml/L group and VitC 50 mg/L group. Nor was there among the GH 500 μmol/L, GH 250 μmol/L and ZnSO4 15 μmol/L groups. The CMV which had been decreased by Ang Ⅱ injury was significantly increased after administrating SMI and GH. The best effect was made by the SMI 10 ml/L group, followed in turn the SMI 5 ml/L, GH 500 μmol/L and GH 250 μmol/L groups. No difference was found among the last two groups. Conclusions SMI, GH, ZnSO4 and VitC could all increase CMV which had been decreased by H2O2 injury, and both SMI and GH could increase CMV which had been decreased by Ang Ⅱ injury.
出处
《中国当代儿科杂志》
CAS
CSCD
2003年第5期443-446,共4页
Chinese Journal of Contemporary Pediatrics
基金
山东省科委课题(No.1999BBIDBAI)
