摘要
采用生物化学方法测定了中华绒螯蟹(Eriocheirsinensis)胚胎发育过程中7个发育期的5种水解酶(类胰蛋白酶、胃蛋白酶、淀粉酶、脂酶和纤维素酶)活力和氨基酸的组成。结果表明,胚胎发育各阶段消化酶活力及氨基酸组成的变化与其发育关系密切。在整个胚胎发育过程中,类胰蛋白酶、胃蛋白酶和纤维素酶的活力呈"高-低-高"的变化趋势,脂酶则呈现逐渐上升的趋势,而淀粉酶的变化趋势不明显。但在原蚤状幼体期,5种酶的活力均显著升高,这与孵化后幼体开口摄食的自身调控机理有关。可溶性蛋白质的含量呈"低-高-低"的变化趋势,明显与相关酶的活力有关。氨基酸含量在前6期变化不大,在原蚤状幼体期显著下降。所有氨基酸中,谷氨酸的含量最高;必需氨基酸中以亮氨酸的含量最高,并且单个必需氨基酸含量与必需氨基酸总量的比值(A/E)在胚胎发育不同阶段略有差异,但基本趋于一致。
The adult Eriocheir sinensis, belonging to Changjiang population and never breeding before, were collected from a crab culture farm in Shanghai. In this experiment, the specific activities of pepsin, tryptase, amylase, lipase and cellulose were determined, as well as their amino acids composition during seven development stages of the embryo. The results indicate that the activities of digestive enzymes and amino acids content closely correlate with the embryonic development stages. During the whole embryonic development of E. sinesis, the activities of pepsin, tryptase and cellulase have a change of 'highlowhigh', while the activity of lipase increases and there is no obvious change in amylase activity. But at the prezoea larval stage, the activities of the five enzymes increase obviously, which has some correlation with the selfadjusting mechanism at the onset of feeding. The content of dissolvable proteins has a trend towards 'lowhighlow', which obviously correlates with the activities of concerned enzymes. The content of amino acids had little change at the first six stages but decreased significantly at the last stage. Among all the amino acids, has Glu the highest content, and has Leu the highest EAA content. There was little difference between the ratios of single EAA content to total EAA content at different embryonic development stages.
出处
《中国水产科学》
CAS
CSCD
北大核心
2003年第5期404-408,共5页
Journal of Fishery Sciences of China
基金
国家自然科学基金(30270161)
教育部骨干教师计划基金
高等学校博士点基金(20010269002).
