摘要
采用组织切片技术,以OlympusBH-2型显微镜对锯缘青蟹(Scyllaserrata)促雄腺的发育进行观察。结果显示,促雄腺附着于射精管近末端,发育过程可分为4期:Ⅰ期促雄腺短小,腺细胞数量少;Ⅱ期促雄腺呈明显的索状;Ⅲ期促雄腺体积达到最大,一些部位膨大增生;Ⅳ期促雄腺不再生长,腺体急剧退化。腺细胞具有2种类型:A型细胞,核圆、异染色质少、胞质染色浅;B型细胞,核扁平、异染色质多,细胞染色深、界限不清晰,有时细胞质消失。促雄腺发育过程中,A、B型细胞数量、比例发生变化。B型细胞为A型细胞完成分泌后的存在形式,B型细胞比例反映了促雄腺分泌活动的状况。锯缘青蟹促雄腺发育和精巢成熟具有一定的相关性。
The male Scylla serrata individuals were employed with body weight 10-618 g and body length 36-94 mm. The observation was conducted under Olympus BH2 microscope. The results show that a pair of androgenic glands attach to the subterminal portion of the ejaculatory ducts, and their developmental process can be divided into four stages: at StageⅠ, the androgenic glands are short and the glandular cells are of small amounts; at Stage Ⅱ, the glands distinctively take on a cord-figure; at Stage Ⅲ, the volume of the glands reaches its peak, with hyperplasia in some portion; at Stage Ⅳ, the glands no longer grow and degenerate rapidly. There are two types of glandular cells: Type A , which has a round nucleus less in heterochromatin and the cytoplasm stained lightly, and Type B, which has flat nucleus rich in heterochromatin, the cytoplasm stained darkly or vanished. During the development of the androgenic gland, the quantity of Type A cells and Type B cells and their ratio in number have some change. After Type A cells perform the secretion function, they exist as the form of Type B cells. At the same development stage of andrgenic gland, the ratio of Type B cells to Type A cells in number increases significantly, which implies that this ratio reflects the secretion activity of the androgenic gland. It can be concluded that androgenic gland correlates closely with the mature degree of the testis.
出处
《中国水产科学》
CAS
CSCD
北大核心
2003年第5期376-380,共5页
Journal of Fishery Sciences of China
基金
福建省重中之重项目资助(1998~2002年)
高等学校博士学科点专项科研基金项目资助(20010384010).
