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旋毛虫死活快速鉴别的实验研究 被引量:17

EXPERIMENTAL STUDY ON DETERMINATION QUICKLY OF VIABILITY OF TRICHINELLA SPIRALIS IN VITRO
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摘要 为了寻找快速鉴别旋毛虫死活的染色方法。用 0 .0 3 %美蓝、美蓝 -伊红 -硼砂染液 (M.E.B)和 1%中性红3种染液 ,对死或活旋毛虫幼虫和成虫进行染色。结果 3种染液均能使死亡旋毛虫幼虫在 1分钟内着色 ,活虫则不着色。 3种染液对旋毛虫死、活成虫均不着色。结论认为 Object: To search for plain and prompt methods in determination of viability of Trichinella spiralis . Methods: The larvae and adult worms of Trichinella spiralis were stained by 0.03% Methylene blue, Methylene blue eosin borax(M.E.B.) and 1% Neutral red, respectively. Results; The dead larvae were stained in one minute and the vital larvae were unstained with all three stains. The vital and dead of adults were unstained in the study. Conclusion: The three stains were plain and prompt methods in determination of the viability of Trichinella spiralis larvae.
出处 《中国兽医寄生虫病》 2003年第4期21-22,共2页 Chinese Journal of Veterinary Parasitology
关键词 旋毛虫 幼虫 成虫 美蓝 M.E.B. 中性红 染色 Trichinella spiralois larva adult Methylene blue M.E.B. Neutral red
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  • 1周炳福 李风玲 梁君谋 等.美蓝、伊红、硼砂染色液鉴别蛔虫卵死活的实验观察[J].寄生虫学与寄生虫病杂志,1985,3(1):48-49.
  • 2何毅勋 毛才生 胡亚青.日本血吸虫尾蚴及童虫的生理学比较[J].寄生虫学与寄生虫病杂志,1983,1(4):15-15.
  • 3IC Wang, YX Ma, CH Kuo, et al. A comparative study on egg hatching methods and oncosphere viability determination for taenia solium eggs[J]. Int J Parasitol, 1997,27 (11 ) : 1311 - 1314.

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