摘要
目的 :探讨沙门菌在腹泻病人粪便、外环境污水和部分动物标本中的菌型分布及其对抗生素的耐药性。方法 :采用血清学方法对沙门菌进行分群和分型 ,采用 K- B法进行抗生素耐药性测定。结果 :4 10份临床腹泻病人标本和 5 14份非临床标本共检出沙门菌 93株 ,检出率为 10 .1% ,其菌型分布为 :腹泻病人粪便中检出的均为 B群鼠伤寒沙门菌 (6 / 93) ,非临床标本中的菌型主要为 C2群纽波特沙门菌 (72 / 93) ,还有 E1群伦敦沙门菌 (7/ 93)、B群鼠伤寒沙门菌 (2 / 93)和未定型 (6 / 93)。 87株沙门菌对青霉素的耐药率最高 (96 .5 % ) ,其次为 SMZ+TMP(73.6 % ) ,未发现对新霉素、链霉素、四环素和阿米卡星的耐药株。结论 :实验结果可为研究我省沙门菌的菌型分布及其对抗生素的耐药性提供参考 ;临床上应加强对鼠伤寒沙门菌引起腹泻的监测。
Objective: To investigate the serotype distribution of Salmonella isolates from feces of diarrhea patients,polluted water and some animals and the resistance of Salmonella isolates to antibiotics.Methods:The group and serotype of Salmonella isolates were determined serologically and the resistance of these isolates to antibiotics was detected by Kirby-Bauer method.Results:93 strains of Salmonella ,which consisted of 72 strains of S.newport of Group C_2 8 strains of S.typhi-murium of Group B,7 strains of S.London of Group E_1 and 6 undetermined strains,were separated from 410 diarrhea patients and 514 non-clinical specimens and the percentage of detection was 10.1%. S.newport of Group C_2 was the dominant serotype among non-clinical isolates and clinincal isolates from diarrhea patients were all S.typhi-murium of Group B.For 87 strains of Salmonella the percentage of resistance was as follows:penicillin G(96.5%),SMZ+TMP(73.6%) erythromycin(49.4%) and carbenicillin (3.5%).The resistant isolates to neomycin,streptomycin,tetracycline and amikacin were not detected.Conclusion:The data above were useful for the research on the serotype distribution of Salmonella and the resistance of Salmonella to antibiotics,and wer should pay attention to monitoring of S.typhi-murium of group B in diarrhea patients epidemically.
出处
《中国微生态学杂志》
CAS
CSCD
2003年第5期287-288,共2页
Chinese Journal of Microecology
关键词
沙门菌
血清型
抗生素
耐药性
Salmonella
Serotype
Antibiotics
Resistance
