摘要
目的 :以体外培养的人牙周膜成纤维细胞 (HPLFs)为研究对象 ,观察IL - 1β对HPLFs的增殖和碱性磷酸酶(ALP)表达的影响 ,旨在从细胞学水平探索IL - 1β在牙周炎的发生、发展、及转归中的作用。 方法 :取酶消化法培养的第 6代细胞 ,随机分 5个实验组及 1个对照组 ,实验组分别加入含系列浓度IL - 1β (0 .1ng/ml、0 .5ng/ml、1ng/ml、5ng/ml、10ng/ml)的α-MEM培养液 ,对照组加入含 1%FBS的α -MEM培养液 ,分别测定MTT和ALP活性 ;加入浓度为 10ng/ml的IL - 1β的α -MEM培养液 ,观察IL - 1β对HPLFs的ALP活性影响的时间效应。 结果 :MTT实验结果表明 ,不同浓度的IL - 1β对HPLFs的增殖与对照组相比无显著差异 (P >0 .0 5 ) ;IL - 1β对HPLFs的HPLFs活性有抑制作用 ,显著低于空白对照组 (P <0 .0 5 ) ;浓度为 10ng/ml的IL - 1β作用于HPLFs,其ALP活性显著低于空白对照组 (P <0 .0 5 )。结论 :IL - 1β在牙周炎的早期阶段可能对HPLFs的增殖无显著影响 ;但在牙周炎的修复过程中IL - 1β可能通过抑制HPLFs的ALP活性 ,阻止HPLFs分化为成骨细胞 。
Objective:The objective of this study were to determine t he effects of IL-1βon human periodontal ligament fibroblasts in culture.Methods:Cell cultures were established from human periodontal lig ament by means of enzyme digestion.Cells were studied at the 6th passage and wer e divided into 5 test groups at random. Each test group was added IL-1β of diff erent concentration (0.1ng/ml,0.5ng/ml,1ng/ml, 5ng/ml, 10ng/ml) in α- MEM mediu m.A group cultured with 1% feral bovine serum was used as a control.Cell prolife ration and alkaline phasphatase activity were determined after the addition of I L-1β.In addition, IL-1β of 10ng/ml was added to the medium and cells were cult ured for 6 days.We determined the alkaline phosphatase activity every day.Results:Cultured in the medium supplemented with different con centration of IL-1β,the proliferation of the HPLFs have no significant differen ces ( P >0.05).However,HPLFs exhibited characteristics consistent with an oste oblast-like phenotype.Compared with the control, cells in medium added with IL- 1βdisplayed a significant decrease in alkaline phosphatase activity depending o n concentration and time( P <0.05).Conclusions:IL-1β pr obably has no significant effect on HPLFs in early periodontitis, but it may pla y an important role in the bone resorption and development of periodontitis.
出处
《口腔医学研究》
CAS
CSCD
2003年第5期356-358,共3页
Journal of Oral Science Research
