摘要
目的通过对三仙汤促进成骨细胞株增殖的研究,判断三仙汤能否应用于绝经后骨质疏松症的防治。方法应用UMR-106成骨细胞株,采用体外细胞培养技术,以氟化钠为阳性对照药,用MTT法测定三仙汤对成骨细胞增殖的影响。结果氟化钠1.25μmol/L、5μmol/L组与空白组相比有显著性差异(前者P<0.01,后者P<0.001);10μmol/L组与空白组无明显差别。三仙液在5、10、100、500、1000mg/L5个不同浓度均使MTT法检测的光吸收值显著升高(P<0.001或P<0.01)。结论三仙汤能促进体外培养成骨细胞增殖。
Objective By studying the influence of Sanxiantang on the proliferation of osteoblast like cells in vitro,to determine if there's a probability of using it in the treatment of menopausal osteoporosis.Methods Culturing the cell strain UMR106 in vitro,using NaF as the control medicine, we observed the effects of Sanxiantang on the proliferation of osteoblast like cells by applying the MTT assay.Results NaF could significantly promoted the proliferation of osteoblast like cells at the concentration of 1.25 μmol/L(P< 0.01),5 μmol/L (P< 0.001), having no significant difference at 10 μmol/L compared with control.While the Sanciantang at the concentrations of 5,10,100,500,1 000 mg/L all could significantly promoted the proliferation of osteoblast like cells in vitro(P< 0.001,P< 0.01).Conclusions Sanxiantang could significantly promote the proliferation of osteoblast like cells in vitro.
出处
《中国临床康复》
CSCD
2002年第7期992-993,共2页
Chinese Journal of Clinical Rehabilitation
